Comparison of Wild Type DNA Sequence of Spike Protein from SARS-CoV-2 with Optimized Sequence on The Induction of Protective Responses Against SARS-Cov-2 Challenge in Mouse Model마우스 모델에서 SARS-CoV-2 공격에 대한 보호 반응 유도에 대한 최적화된 서열과 SARS-CoV-2의 스파이크 단백질 야생형 DNA 서열의 비교Article Published on 2022-12-312022-09-11 Journal: Human vaccines & immunotherapeutics [Category] COVID19(2023년), SARS, 비임상, 진단, [키워드] acid Against Antiviral binding breath CD4 CD8+ cellular immune responses cellular immunity challenge clinical trial contributing to COVID-19 CTL demonstrated DNA DNA vaccine expression full-length spike protein hACE2 humoral humoral and cellular immunity IFN-γ immune immune responses immunogen in vitro induction mice Model mouse Neutralizing antibodies optimization optimizations pGX-9501 platform Protective Protective effects protective response RBD binding response robust SARS-CoV-2 sequence spike Spike protein subsequent T cell T cells the vaccine Transgenic mouse Type virus wild-type wild-type sequence. [DOI] 10.1080/21645515.2021.2016201 PMC 바로가기 [Article Type] Article
Generation of Angiotensin-Converting Enzyme 2/Transmembrane Protease Serine 2-Double-Positive Human Induced Pluripotent Stem Cell-Derived Spheroids for Anti-Severe Acute Respiratory Syndrome Coronavirus 2 Drug EvaluationArticle Published on 2022-12-212023-07-10 Journal: Microbiology Spectrum [Category] COVID19(2023년), [키워드] COVID-19 hACE2 hiPSC-derived spheroids in vitro drug evaluation. SARS-CoV-2 TMPRSS2 [DOI] 10.1128/spectrum.03490-22 PMC 바로가기
Identification and characterization of the anti-SARS-CoV-2 activity of cationic amphiphilic steroidal compounds양이온성 양친매성 스테로이드 화합물의 항 SARS-CoV-2 활성의 확인 및 특성화Article Published on 2022-12-012022-09-11 Journal: Virulence [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료제, [키워드] aggravation ameliorated anti-SARS-CoV-2 activities Anti-SARS-CoV-2 Activity Antiviral antiviral activity cationic caused cell lines characterization cholesterol complex Compound compounds COVID-19 pandemic COVID-19 patient cytopathic Cytopathic effect Drug discovery effective envelope envelope proteins epiandrosterone followed by hACE2 HEK293 host cell host cells identification in vitro in vivo indicated inhibited initial involved life Lipid mechanism membrane modulate modulation Mortality Pathogenesis progeny protective effect Protective effects Proteins Quality of life reduce reduce COVID-19 aggravation SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication selected Selection single mutation single mutations specific treatment spike steroidal compounds. subversion Transgenic mouse translate transmembrane Treatment treatment of COVID-19 patients Vero Vero CCL81 cell Vero CCL81 cells viral replication Viral RNA viral variant Viral variants [DOI] 10.1080/21505594.2022.2085793 PMC 바로가기 [Article Type] Article
Lucidenic acid A inhibits the binding of hACE2 receptor with spike protein to prevent SARS-CoV-2 invasionArticle Published on 2022-11-012022-11-15 Journal: Food and chemical toxicology : an international jo [Category] SARS, 변종, 치료제, [키워드] analyzed anti-SARS-CoV-2 antiviral effects binding caused complexes component components demonstrated exhibit FRET test Ganoderma lucidum triterpenoids hACE2 hACE2 receptor high affinity hydrogen bonds IC50 in vitro Infection inhibit inhibited inhibition assay Invasion Lucidenic acid A (PubChem CID: 14109375) molecular docking molecular dynamics Mutation natural natural products omicron Omicron variant omicron variant. pathway Potential Prevent PubChem receptor receptor proteins SARS-CoV-2 Spike protein stability target variant Virtual screening virus was selected were used [DOI] 10.1016/j.fct.2022.113438 PMC 바로가기
In silico analysis of SARS-CoV-2 spike protein N501Y and N501T mutation effects on human ACE2 bindingSARS-CoV-2 스파이크 단백질 N501Y 및 N501T 돌연변이가 인간 ACE2 결합에 미치는 영향에 대한 실리코 분석Article Published on 2022-11-012022-09-11 Journal: Journal of molecular graphics & modelling [Category] COVID19(2023년), SARS, 변종, [키워드] Analysis analyzed anti-SARS-CoV-2 antibodies Anti-viral drugs antisense attachment binding binding energy binding free energy classic Dynamics Effect Effect of mutations on binding energy effective free energy hACE2 hACE2 receptor hACE2-SARS-CoV-2 spike protein binding. handling highlight Host human ACE2 maintain molecular molecular dynamics molecular dynamics simulations Mutation N501Y N501Y and N501T mutations nine outcome predicted Protein binding raised Replication SARS CoV SARS-CoV-2 SARS-CoV-2 mutations SARS-CoV-2 spike protein Spike protein survival Transmissibility treatment strategy Vaccines virus [DOI] 10.1016/j.jmgm.2022.108260 PMC 바로가기 [Article Type] Article
Identification of hACE2-interacting sites in SARS-CoV-2 spike receptor binding domain for antiviral drugs screeningArticle Published on 2022-11-012022-11-15 Journal: Virus research [Category] SARS, 변종, 치료제, [키워드] amino acid residue antiviral drug binding binding sites. Cefixime Ceftazidime cell entry Cephalosporin derivative clinical evaluation coronavirus 2 Critical derivative drug Drug screening example hACE2 human Angiotensin-converting enzyme identification Infection inhibited Interaction key amino acid live SARS-CoV-2 molecular docking Mutation peptide Pseudotyped virus pseudovirus RBD Receptor binding domain respiratory Safe SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 Spike RBD Support targets tested the RBD the spike protein variants virus was determined wild type wildtype Wuhan [DOI] 10.1016/j.virusres.2022.198915 PMC 바로가기
SARS-CoV-2 tetrameric RBD protein blocks viral infection and induces potent neutralizing antibody responseArticle Published on 2022-10-312023-07-10 Journal: Frontiers in Immunology [Category] COVID19(2023년), [키워드] 2xS-RBD-mFc hACE2 SARS-CoV-2 Vaccine Viral infection. [DOI] 10.3389/fimmu.2022.960094 PMC 바로가기
De novo selected hACE2 mimics that integrate hotspot peptides with aptameric scaffolds for binding tolerance of SARS-CoV-2 variantsArticle Published on 2022-10-282022-11-15 Journal: Science Advances [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] acute respiratory syndrome Antiviral binding coronavirus Critical de novo evade exhibited hACE2 host cell hotspot human Angiotensin-converting enzyme immune in vitro Interaction Ligand Neutralizing nucleic acids Occurrence omicron peptide Prophylaxis random reagents Receptor binding receptors recruit SARS-CoV-2 SARS-CoV-2 variant selected Tolerance Vaccine variant variants viral variant virus [DOI] 10.1126/sciadv.abq6207 PMC 바로가기
Monitoring SARS-CoV-2 Infection Using a Double Reporter-Expressing VirusArticle Published on 2022-10-262022-11-15 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome animal models antiviral therapeutics antivirals biology of SARS-CoV-2 Care caused comparable contagious coronavirus coronavirus disease COVID-19 cultured cells demonstrated detect Express expresse expressing expression level facilitate feasibility fluorescent Genes hACE2 hamster Health human Angiotensin-converting enzyme in vitro In vitro assay in vivo infected cell Infection K18 knowledge limitation luciferase luciferase reporter gene luciferase reporter genes mCherry monitoring neutralizing antibody overcome pandemic pathogenicity Protein recombinant reporter responsible robust SARS-CoV-2 SARS-CoV-2 biology SARS-COV-2 infection transgenic mice Transmission Transmission. Vaccine viral fitness viral infection virus wild-type [DOI] 10.1128/spectrum.02379-22 PMC 바로가기
Structural insights into the binding of SARS-CoV-2, SARS-CoV, and hCoV-NL63 spike receptor-binding domain to horse ACE2Article Published on 2022-10-062022-11-16 Journal: Structure (London, England : 1993) [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 acute respiratory syndrome affinity Analysis B.1.617.2 Beta bind binding binding affinity cell entry complex complex structure coronavirus coronavirus Coronaviruses Delta Efficiency equine Equus functional Gamma hACE2 HCoV-NL63 HeLa cells high affinity horse human ACE2 indicated Infection infection assay molecular basis Mutation provide pseudovirus pseudovirus infection RBDs receptor Receptor binding domain receptor binding domain. Receptor-binding domain SARS-CoV SARS-CoV-2 SARS-CoV-2 variant significantly increased spike receptor-binding domain the RBD the SARS-CoV-2 the spike protein Transmission zoonotic [DOI] 10.1016/j.str.2022.07.005 PMC 바로가기